Efficacy

Evaluation Model

Evaluation Indicator

Anti-wrinkle

Elastase inhibition experiment

Elastase activity inhibition rate

Collagen inhibition experiment

Inhibition rate of collagenase

UVA-induced model of primary fibroblast aging

Measurement of ROS content, staining of β-galactosidase, and mitochondrial membrane potential

UVA-induced model of primary fibroblast aging

Type I collagen and elastin content

UVA-induced model of primary fibroblast aging

Expression of elastin degradation related genes (MMPs)

UVA-induced model of primary fibroblast aging

Expression of aging-related genes (P53, P21, P16)

Firmness

Elastase inhibition experiment

Elastase activity inhibition rate

Primary fibroblast aging model

Type I collagen and elastin content

Cell-aging Model

UVB-induced photoaging model in epidermal stem cells

Measurement of ROS content, staining ofβ-galactosidase, mitochondrial membrane potential and stemness (integrinα6 and integrin β1) expression

UVA-induced model of fibroblast aging

Measurement of ROS content and expression of aging-related genes (P53, P21, P16)

Advanced glycation end products (AGEs) induced model of primary fibroblast aging

Measurement of ROS content and expression of aging - related genes (P53, P21, P16)

Anti-oxidant

DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay

DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay free radical scavenging rate

PTIO (2-phenyl- 4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide) radical scavenging assay

PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide) free radical scavenging rate

Induction of oxidation stress model in HaCaT by UVB

Free radicals including ROS, SOD

Anti-glycosylation

Bovine serum albumin (BSA) - Fructose Maillard reaction simulation

Inhibition rate of advanced glycation end products (AGEs)

High glucose-induced fibroblast glycation model

AGEs (CML), measurement of RAGE content, and measurement of glucose uptake

Glycation model of natural aging in primary fibroblasts

AGEs (CML), measurement of RAGE content