Efficacy

Evaluation Model

Evaluation Indicator

Acne removal

Measurement of sebum content in sebaceous gland cells

Lipid droplets

Stimulation of sebum secretion in sebaceous gland cells by sodium palmitate

Lipid droplets, Expression of genes related to lipid synthesis (SREBP-1, FAS)

Stimulation of sebum secretion in sebaceous gland cells by dihydrotestosterone (DHT)

Lipid droplets, Expression of genes related to lipid synthesis (SREBP-1, FAS)

LPS stimulated inflammation model in HaCaT cells

Measurement of inflammatory factors (IL-6, IL-8, TNF-α, IL-β1, etc.)

Propionibacterium acnes bacteriostasis experiment

Bacterial growth inhibition zone, MIC

Measurement of bacterial biofilm formation

Inhibition rate of bacterial colonization

Oil control

Measurement of sebum content in sebaceous gland cells

Lipid droplets

Stimulation of sebum secretion in sebaceous gland cells by sodium palmitate

Lipid droplets, Expression of genes related to lipid synthesis (SREBP-1, FAS)

Stimulation of sebum secretion in sebaceous gland cells by dihydrotestosterone (DHT)

Lipid droplets, Expression of genes related to lipid synthesis (SREBP-1, FAS)

Type II 5α-reductase inhibition experiment

Inhibition rate of dihydrotestosterone (DHT) generation